The JUNIOR-PAM fluorometer is a miniaturized instrument capable to carry out the full range of saturation pulse analysis of photosystem II. A small box of 11.5 x 6.5 x 3.0 cm contains all optical and electronic components. The fluorometer is connected to the sample via a 50 cm long single plastic fiber with 1.5 mm diameter.
The JUNIOR-PAM employs the same blue (460 nm) power LED for pulse modulated fluorescence excitation, actinic illumination and saturating pulses. Also, the fluorometer features a far-red LED for selective excitation of PS I and determination of the Fo’ level fluorescence.
Two different types of leaf-clips are provided, a 60° clip for measurements in ambient light and a magnetic leaf clip for Fo-Fm and Fv/Fm measurements.
Specifications
Optoelectronic Unit JUNIOR-PAM
(BLUE Version)
Modulated fluorescence excitation: Blue LED (wavelength of maximum emission: 445 nm). Modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz, and 100 Hz. Fluorescence at wavelengths greater than 630 nm is measured
Actinic light: Same LED as used for modulated light. PAR at 1 mm distance from the tip of the 50 cm JUNIOR-PAM light guide: 25 to 1500 μmol m-2 s-1. Maximum PAR of saturation pulses, 10 000 μmol m-2 s-1
Optoelectronic Unit JUNIOR-PAM/W
(WHITE Version)
Modulated fluorescence excitation: White LED (wavelengths of maximum emission: 445 and 545 nm). Modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz, and 100 Hz. Fluorescence at wavelengths greater than 650 nm is measured
Actinic light: Same LED as used for modulated light. PAR at 1 mm distance from the tip of the 50 cm JUNIOR-PAM light guide: between 25 and 1500 μmol m-2 s-1. Maximum PAR of saturation pulses, 10 000 µmol m-2 s-1
Optoelectronic Unit JUNIOR-PAM
and JUNIOR-PAM/W
Housing: Aluminum housing with USB type B socket, M8 4-pole socket to connect the JUNIOR-PAM monitoring leaf clip (accessory), port for JUNIOR-PAM light guide, and swivel-mounted sample support
Far-red light: LED with 745 nm maximum emission wavelength for selective excitation of photosystem I
Fluorescence detection: PIN photodiode protected by long-pass filter. Selective window amplifier to measure pulse-amplitude modulated (PAM) fluorescence
Communication: Standard USB communication via 1.2 m USB-cable type A-B
Power supply: 5 V DC supplied by the USB-cable type A-B used for communication
Power consumption: 100 mW at normal operation and 500 mW during saturation pulse
Dimensions: 11.5 cm x 6.5 cm x 3 cm (L x W x H)
Weight: 200 g
Operating temperature: 0 to + 40 °C
Operating humidity range: 35 to 85% RH (to avoid condensation)
Software WinControl-3
Program: WinControl-3 System Control and Data Acquisition Program (Microsoft Windows XP/Vista/7/8/10) for operation of measuring system via PC, data acquisition and analysis
Saturation Pulse Analysis: Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. PAR, leaf temperature and relative humidity using Monitoring Leaf-Clip JUNIOR-BD. Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)
Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves
Programmability: Fully programmable using batch file language
Computer Requirements: 1 free USB socket. Processor, 1 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0
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